Yes, you can. I am surprised that you would ask.
OrCAD PSpice, Cadence HSPICE in Spectre/Virtuoso/Analog Design Environment, etc, all equipped with this basic functional capability to simulate transiences of mixed-signal analogue-digital circuits and systems, however only at one input frequency from a clock source or oscillator.
For RF and Microwave, you need to run at "stepped frequency" on Spice. Ansoft has this capability. The latest HP-ADS too, has this capability.