I know this, my question is that assumes conditons that we use this data for other blocks,how you can find out that this value is two complementary or not?
If you have defined the signed reg or wire and , you do not have to worry about then, If it is negative value, It is 2's compliment, e.g. 111010 for -5 and 000101 for +5
even if you are using it in any way.
Try making a simple project of adder etc. with signed regs and wires and test it.