are you using an STK200 (or AVR isp) programmer??? that one on the parallel port?????
if you use an STK200 on the LPT1, have you installed the GiveIO driver (that one on the top-right?)
If all the software is configured, you have a hardware problem...
- are you using a 5v power supply on the AVR (don't spect a simple STK200 give power to the avr)
- have you checked all the wires? they are 5!
- maybe the MOSI/MISO interchange problem (check it! dont switch places at free will!)