How to use GUI data in other functions in MATLAB ?

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Hello! Everyone
I am trying to make a GUI, which periodically sends some packet over Serial Port.
I need to send this packet 10 times, and if there is no response from Serial Port the timeout will occur.
I made a variable *handles.TimeOutCounter* in the Opening Function, with the intention that, i will increment it every time and reset it to zero whenever there is data from Serial Port.
Currently i haven't implemented the Serial Reception CallBack function yet, so this function must transmit the data for 10 times, but it is sending data just one time and after that it gives some error.

_Error while evaluating TimerFcn for timer 'timer-1'

H must be the handle to a figure or figure descendent._

The code is as follow:

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Code Visual Basic - [expand]
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% --- Executes just before UltraSonicSensor is made visible.
  function UltraSonicSensor_OpeningFcn(hObject, eventdata, handles, varargin)
  % This function has no output args, see OutputFcn.
  % hObject    handle to figure
  % eventdata  reserved - to be defined in a future version of MATLAB
  % handles    structure with handles and user data (see GUIDATA)
  % varargin   command line arguments to UltraSonicSensor (see VARARGIN)
  
  % Choose default command line output for UltraSonicSensor
  handles.output = hObject;
  axes(handles.axes1)
  [SensorImage, SensorMap] = imread('hc-sr04.bmp');
  imshow(SensorImage, SensorMap)
  axis off
  axis image
  
  % Delete any opened Ports
  delete(instrfind);
  % Create Serial Object at 9600 BaudRate
  handles.serial = serial('COM1','BaudRate', 9600);
  handles.serial.BytesAvailableFcnMode = 'terminator';
  % Serial Reception Callback Funtion
  handles.serial.BytesAvailableFcn = @Serial_Receive;
  % Open Serial Port
  fopen(handles.serial);
  
  % Counter Variable
  handles.TimeOutCounter = 1;
  % Delete any previously defined timer
  delete(timerfind);
  % Starts a 1 second timer
  handles.timer = timer;
  handles.timer.TimerFcn = {@Timer_1Second, handles};
  handles.timer.StartDelay = 5;
  handles.timer.ExecutionMode = 'FixedRate';
  start(handles.timer);
  % Update handles structure
  guidata(hObject, handles);
  
  % UIWAIT makes UltraSonicSensor wait for user response (see UIRESUME)
  % uiwait(handles.GUI1);
  
  
  % --- Outputs from this function are returned to the command line.
  function varargout = UltraSonicSensor_OutputFcn(hObject, eventdata, handles) 
  % varargout  cell array for returning output args (see VARARGOUT);
  % hObject    handle to figure
  % eventdata  reserved - to be defined in a future version of MATLAB
  % handles    structure with handles and user data (see GUIDATA)
  
  % Get default command line output from handles structure
  varargout{1} = handles.output;
  
  % Function gets called after 1 second
  function Timer_1Second(hObject, evendata, handles)
  handles.output = hObject;
  % Construct Packet to Send
  My_Address = 14;        % 0x0E
  Trans_Header = 44;      % 0x2C
  Trans_Destination = 36; % 0x24
  Trans_Source = My_Address;
  Trans_Length = 2;
  Trans_OpCode = 1;
  Trans_Data = 1;
  data = [Trans_Header,Trans_Destination,Trans_Source,Trans_Length ...
      ,Trans_OpCode,Trans_Data];
  % Computer Checksum
  Trans_Checksum = Compute_Checksum(data,6);
  % Append Checksum at the end of the Packet
  data(end+1) = Trans_Checksum;
  % Check whether the Serial Port is Open
  if strcmp(get(handles.serial,'Status'),'open')
      % Send data over Serial Port
      fwrite(handles.serial,data,'uchar');
      % Increment Counter Variable
      handles.TimeOutCounter = handles.TimeOutCounter + 1;
      % Debugging Purpose, Print the value on Serial Port
      fprintf('Value is = %d\n ',handles.TimeOutCounter);
  end
  
  if handles.TimeOutCounter > 10
      % Timeout Error, No Data receive in desired time.
      stop(handles.timer);
      fclose(handles.serial);
      delete(instrfind);
  end
  % Update handles structure
  guidata(hObject, handles);

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Hope you all understands my intention, what i want to do.
Please help me, i am stuck in this problem last few days.