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You didn't tell about the used instrumentation amplifier or the circuit, but what's the reason for output offset? If the instr-amp is operated from single supply, it may be trivial output saturation. Then shifting the output won't help much.
Datasheet figures 5 and 6 are discussing different kinds of single supply operation. I don't know which is better meeting your application. If you need a bipolar output signal, then IAref need to be connected to a positive "pseudoground" or virtual ground as in figure 6. The output will have an offset, but you can hardly remove it without losing the bipolar signal swing. If your signal is unipolar, but the signal has an unwanted offset, which would be originated from the input, an additional differential amplifier, as suggested by Alex, can help. But you should consider, that no single supply amplifier can achieve exactly zero output.
Another imnportant point with instrumentation amplifiers is keeping the input common mode range. Related to single supply operation of INA125 this implies a positive bias voltage to be applied to the inputs.
Sorry for late a update, i am having problem to take output signal for offset removing circuit as input signal.Basically i am using INA125 with single supply and if i check output from pin no. 11 with reference with pin no.5 i am having offset voltage of +1.4v if i check output from pinno.11 and with reference ground its show output as 3.57v(whether offset i don't know)so how i can take signal for my offset removing circuit?????
hi I have the same problem with the ina125 I don´t know how to remove the offset, in the out I have 0.05 V when at the entry I have 0 V, I don´t know how I cn use the vref pin please help me I´m using the amplifier eith a single supply
thanks for your help