isit possile to amplifier asignal twice? i have a source range between 0.2V-0.25V. would like to amplifier it to provide output of 1-5V. But i only manage to amplifer it 10x meaning 2-2.5V.. can i amplifier this again to get output between 1-5V only?
What you need is to amplify the signal 80 times, since your input range is only 50mV, from 0.2 to 0.25V. Then you subtract an offset to make the output range 1~5V.
Take a look at this circuit. It should do what you need. Adjust the 0.19V reference to get 1V at output with 0.2V input and adjust one of the resistors to get 5V with 0.25V input.