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I assume you're considering a differential amplifier here, due to approximations and assumptions made theoretically (considering a small input signal) your gain should be constant over all input range, as long as you're working far from the saturation and cut-off edges for your amplifier.
If this isn't quite what you were expecting as an aswer then kindly do clear out your question and ISA I will try to look it up for you.
I disagree with the above response. The gain will not be constant over your command mode range and it is thus necesary to run ac sims over a variety of points. In addition, you should run transient sims using step functions for the output load, input voltage, and vdd voltage to ensure stability over the entire common mode range. This is a necessary step when running stability sims because the poles and zeros of your system can change drastically with large signal changes and ac sims do not account for this phenomenon.
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