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particle analysis on an image in MATLAB

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Engr.KamranHameed

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Hi all,
I am trying to do particle analysis on an image in MATLAB.I work with nanocrystals of varying sizes and contrast. Basically what I am trying to do is count the number of cells in the image by setting thresholds and make measurements like area, perimeter, radius. The challenge with this work is that the nano crystals change both in contrast and circularity from image to image.
Has anybody worked on this before? Does anybody know what algorithm works best in this case.
Please let me know. It will really help me.

Thanks,
 

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